Hotspot

trnF-GAA-ndhJ

LSC · 51468-52221 · 754 bp

Back to Syzygium

Pi 0.0112

MarkerSeek score 59.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 136
pi: 0.0112
unique_haplotype_count: 111
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1296-1358 bp	1.0	91.8
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GTGCGGATTTTCAAGAACGG	55.0	50.0	58.0	58.0	976-1038 bp	0.994318	91.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.1	58.0	1260-1322 bp	1.0	91.4
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	TGGATTTGGAAAAGTGCGGA	55.0	45.0	58.0	58.0	989-1051 bp	0.988636	91.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATTTGGAAAAGTGCGGA	50.0	45.0	58.1	58.0	953-1015 bp	0.988636	91.0