Hotspot

petN-psbM

LSC · 30574-31543 · 970 bp

Back to Syzygium

Pi 0.00812

MarkerSeek score 73.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 177
pi: 0.00812
unique_haplotype_count: 131
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	1070-1145 bp	1.0	76.2
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	1076-1151 bp	1.0	74.7
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1016-1091 bp	0.994318	73.2
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	1070-1145 bp	1.0	71.8
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	50.0	45.0	57.8	55.5	1076-1151 bp	1.0	70.3