Hotspot

trnS-GCU-trnG-UCC

LSC · 8901-9694 · 794 bp

Back to Syzygium

Pi 0.00979
MarkerSeek score 63.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
794
variable_sites
162
pi
0.00979
unique_haplotype_count
139
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA ACGAATCCCACTTTTACCACT 50.0 42.9 58.2 57.2 785-948 bp 1.0 76.5
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA AACGAATCCCACTTTTACCACT 50.0 40.9 58.2 57.9 786-949 bp 1.0 74.0
trnS-GCU-trnG-UCC_p3 AACGGAAAGAGAGGGATTCG ACGAATCCCACTTTTACCACT 50.0 42.9 57.3 57.2 786-949 bp 1.0 72.3
trnS-GCU-trnG-UCC_p4 ACGGAAAGAGAGGGATTCGA ACGAATCCCACTTTTACCAC 50.0 45.0 58.2 55.7 785-948 bp 1.0 72.1
trnS-GCU-trnG-UCC_p5 CTTTCGTCCACTCAGCCATC ACGAATCCCACTTTTACCACT 55.0 42.9 58.6 57.2 721-879 bp 0.943182 70.4