Hotspot

ycf4

LSC · 62504-63073 · 570 bp

Back to Syringa

Pi 0.0558

MarkerSeek score 63.70

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 570
variable_sites: 91
pi: 0.0558
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	TGGCTCGACTTAACGTTCTG	45.0	50.0	58.0	57.9	1118-1282 bp	1.0	91.3
ycf4_p2	ATTTCCGGCAATTGCAATGG	CCTCATTCGTCGGCTCTATG	45.0	55.0	58.0	57.9	1751-1922 bp	1.0	89.9
ycf4_p3	GGATCGCTGGATGGCTAAAA	CCTCATTCGTCGGCTCTATG	50.0	55.0	58.0	57.9	1532-1667 bp	1.0	87.6
ycf4_p4	ATTTCCGGCAATTGCAATGG	TCGTCGGCTCTATGTGATCT	45.0	50.0	58.0	58.0	1745-1916 bp	0.806452	82.2
ycf4_p5	GGATCGCTGGATGGCTAAAA	TCGTCGGCTCTATGTGATCT	50.0	50.0	58.0	58.0	1528-1661 bp	0.806452	79.9