Hotspot

ycf2

IRb · 89396-95293 · 5898 bp

Back to Syringa

Pi 0.0198

MarkerSeek score 51.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 5898
variable_sites: 369
pi: 0.0198
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCGCTTATGGGGTCAAATCA	AGATCTCGTGCATTGGAACC	50.0	50.0	57.9	58.0	1189-1369 bp	1.0	91.4
ycf2_p2	ATTGAACAACCGGGAGCAAT	AGATCTCGTGCATTGGAACC	45.0	50.0	58.1	58.0	845-1016 bp	1.0	91.1
ycf2_p3	AAATCGAATCCTGGGTCGTG	AGATCTCGTGCATTGGAACC	50.0	50.0	58.0	58.0	1006-1116 bp	0.387097	61.0
ycf2_p4	AAATCGAATCCTGGGTCGTG	GGTTTAGTTCCGGCAGCATA	50.0	50.0	58.0	58.0	699-740 bp	0.16129	46.0
ycf2_p5	CCGCTTATGGGGTCAAATCA	GGTTTAGTTCCGGCAGCATA	50.0	50.0	57.9	58.0	882-923 bp	0.16129	45.8