Hotspot

accD

LSC · 59403-61295 · 1893 bp

Back to Syringa

Pi 0.0964

MarkerSeek score 65.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1893
variable_sites: 484
pi: 0.0964
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCGGATTCCCACAAAAGAA	GCCGGAAATACTAGGCCTAC	50.0	55.0	58.1	57.5	2397-2906 bp	0.774194	78.7
accD_p2	CCCGGATTCCCACAAAAGAA	CGACCCAACAGGAAGGAATT	50.0	50.0	58.1	57.8	2320-2500 bp	0.387097	61.0
accD_p3	AGTGAAGTCTTACCCCGGAT	CGACCCAACAGGAAGGAATT	50.0	50.0	57.8	57.8	2333-2513 bp	0.387097	59.6
accD_p4	TGAAAACTCGGCCCAATCTT	CGACCCAACAGGAAGGAATT	45.0	50.0	57.7	57.8	2800-2977 bp	0.387097	58.7
accD_p5	TTGAAAACTCGGCCCAATCT	CGACCCAACAGGAAGGAATT	45.0	50.0	57.7	57.8	2801-2978 bp	0.387097	58.7