Hotspot

rpl32-trnL-UAG

SSC · 116223-117082 · 860 bp

Back to Syringa

Pi 0.0249

MarkerSeek score 60.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 860
variable_sites: 87
pi: 0.0249
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTTGCCGTCGGAAAAAGAAG	AAAAATAGCACTTGGGCCGA	50.0	45.0	58.0	58.1	1984-2115 bp	1.0	89.2
rpl32-trnL-UAG_p2	TGCCGTCGGAAAAAGAAGAA	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.0	58.1	1982-2113 bp	1.0	89.1
rpl32-trnL-UAG_p3	GTTGCCGTCGGAAAAAGAAG	TGTCCCGAATAAATCCAGCG	50.0	50.0	58.0	58.1	1946-1967 bp	0.677419	71.4
rpl32-trnL-UAG_p4	TGCCGTCGGAAAAAGAAGAA	TGTCCCGAATAAATCCAGCG	45.0	50.0	58.0	58.1	1944-1965 bp	0.677419	71.4
rpl32-trnL-UAG_p5	TTGCCGTCGGAAAAAGAAGA	TGTCCCGAATAAATCCAGCG	45.0	50.0	58.0	58.1	1945-1966 bp	0.677419	71.4