Hotspot

rbcL-accD

LSC · 58656-59402 · 747 bp

Back to Syringa

Pi 0.0607

MarkerSeek score 71.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 157
pi: 0.0607
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	ACGACCCCTCCAACTATGAA	50.0	50.0	58.0	58.1	1056-1420 bp	1.0	91.5
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	ACGACCCCTCCAACTATGAA	50.0	50.0	58.0	58.1	1535-1899 bp	1.0	91.4
rbcL-accD_p3	TCTTCACATTCACCGTGCAA	ACGACCCCTCCAACTATGAA	45.0	50.0	58.0	58.1	1383-1747 bp	1.0	91.3
rbcL-accD_p4	TTCTTCACATTCACCGTGCA	ACGACCCCTCCAACTATGAA	45.0	50.0	58.0	58.1	1384-1748 bp	1.0	91.3
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	ACGACCCCTCCAACTATGAA	55.0	50.0	58.0	58.1	962-1008 bp	0.774194	78.2