Hotspot

psaA-ycf3

LSC · 43472-44197 · 726 bp

Back to Syringa

Pi 0.0205

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 76
pi: 0.0205
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	GGAAACAGGCTATAGCGCTT	61.1	50.0	58.4	58.0	819-1572 bp	1.0	71.0
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGG	61.1	50.0	58.4	57.9	771-1524 bp	1.0	70.8
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCAGCTGA	61.1	45.0	58.4	57.7	844-1597 bp	1.0	69.1
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	AGGCTATAGCGCTTACTCCT	61.1	50.0	58.4	57.7	813-1566 bp	1.0	69.1
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	TTGGAAACAGGCTATAGCGC	61.1	50.0	58.4	58.3	821-1574 bp	1.0	68.7