Hotspot

psbZ-trnG-GCC

LSC · 37783-38110 · 328 bp

Back to Syringa

Pi 0.0336
MarkerSeek score 69.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
328
variable_sites
47
pi
0.0336
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 478-512 bp 1.0 89.7
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 479-513 bp 1.0 89.7
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 476-510 bp 1.0 89.3
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 477-511 bp 1.0 89.3
psbZ-trnG-GCC_p5 GGGTTAGTCTTTCTGGTGGG AATCGAACCCGCATCTTCTC 55.0 50.0 57.5 58.1 413-447 bp 1.0 87.6