primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	57.9	53.8	0.0	0.0	4.374141	89	519	312-518	1.000000	57.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACTGCCTTAATCCACTTGGC	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	58.2	53.8	0.0	0.0	4.399928	89	519	313-519	1.000000	56.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTTGGCTACATCCG	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	57.7	53.8	0.0	0.0	4.571415	89	519	305-511	1.000000	55.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GCCTTAATCCACTTGGCTACA	GAAGCTCCATCTACAAATGG	21	20	47.6	45.0	58.0	53.8	0.0	0.0	5.240021	89	519	310-516	1.000000	52.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TGCCTTAATCCACTTGGCTAC	GAAGCTCCATCTACAAATGG	21	20	47.6	45.0	58.0	53.8	0.0	0.0	5.240021	89	519	311-517	1.000000	52.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATTCCTCGCTTCATCCA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.1	58.2	0.0	1.3	0.250342	4380	5264	1045-1062	1.000000	85.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4380	5264	997-1014	1.000000	85.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4380	5264	996-1013	1.000000	85.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.3	58.2	0.0	1.3	0.456153	4380	5264	1011-1028	1.000000	84.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.3	58.2	5.3	1.3	0.473122	4380	5264	1006-1023	1.000000	84.2
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37783	38110	478-512	1.000000	89.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	37783	38110	479-513	1.000000	89.7
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37783	38110	476-510	1.000000	89.3
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	37783	38110	477-511	1.000000	89.3
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	57.5	58.1	0.0	0.0	0.550350	37783	38110	413-447	1.000000	87.6
psaA-ycf3_p1	psaA-ycf3	1	CTTCTGGTTCCGGCGAAC	GGAAACAGGCTATAGCGCTT	18	20	61.1	50.0	58.4	58.0	9.3	18.7	2.482438	43472	44197	819-1572	1.000000	71.0
psaA-ycf3_p2	psaA-ycf3	2	CTTCTGGTTCCGGCGAAC	ATTGGTTGAAGATCACGGGG	18	20	61.1	50.0	58.4	57.9	9.3	0.0	2.566136	43472	44197	771-1524	1.000000	70.8
psaA-ycf3_p3	psaA-ycf3	3	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCAGCTGA	18	20	61.1	45.0	58.4	57.7	9.3	20.4	2.712499	43472	44197	844-1597	1.000000	69.1
psaA-ycf3_p4	psaA-ycf3	4	CTTCTGGTTCCGGCGAAC	AGGCTATAGCGCTTACTCCT	18	20	61.1	50.0	58.4	57.7	9.3	18.7	2.733438	43472	44197	813-1566	1.000000	69.1
psaA-ycf3_p5	psaA-ycf3	5	CTTCTGGTTCCGGCGAAC	TTGGAAACAGGCTATAGCGC	18	20	61.1	50.0	58.4	58.3	9.3	18.3	2.773671	43472	44197	821-1574	1.000000	68.7
rbcL-accD_p1	rbcL-accD	1	ACTACAGTTCGGTGGAGGAA	ACGACCCCTCCAACTATGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.058386	58656	59402	1056-1420	1.000000	91.5
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	ACGACCCCTCCAACTATGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.071557	58656	59402	1535-1899	1.000000	91.4
rbcL-accD_p3	rbcL-accD	3	TCTTCACATTCACCGTGCAA	ACGACCCCTCCAACTATGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.092599	58656	59402	1383-1747	1.000000	91.3
rbcL-accD_p4	rbcL-accD	4	TTCTTCACATTCACCGTGCA	ACGACCCCTCCAACTATGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.092599	58656	59402	1384-1748	1.000000	91.3
rbcL-accD_p5	rbcL-accD	5	GAGGCTAGCAAATGGAGTCC	ACGACCCCTCCAACTATGAA	20	20	55.0	50.0	58.0	58.1	18.2	0.0	0.092599	58656	59402	962-1008	0.774194	78.2
accD_p1	accD	1	CCCGGATTCCCACAAAAGAA	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	58.1	57.5	0.7	8.9	0.534423	59403	61295	2397-2906	0.774194	78.7
accD_p2	accD	2	CCCGGATTCCCACAAAAGAA	CGACCCAACAGGAAGGAATT	20	20	50.0	50.0	58.1	57.8	0.7	0.0	0.282501	59403	61295	2320-2500	0.387097	61.0
accD_p3	accD	3	AGTGAAGTCTTACCCCGGAT	CGACCCAACAGGAAGGAATT	20	20	50.0	50.0	57.8	57.8	0.7	0.0	0.449682	59403	61295	2333-2513	0.387097	59.6
accD_p4	accD	4	TGAAAACTCGGCCCAATCTT	CGACCCAACAGGAAGGAATT	20	20	45.0	50.0	57.7	57.8	0.0	0.0	0.486737	59403	61295	2800-2977	0.387097	58.7
accD_p5	accD	5	TTGAAAACTCGGCCCAATCT	CGACCCAACAGGAAGGAATT	20	20	45.0	50.0	57.7	57.8	0.0	0.0	0.486737	59403	61295	2801-2978	0.387097	58.7
ycf4_p1	ycf4	1	ATTTCCGGCAATTGCAATGG	TGGCTCGACTTAACGTTCTG	20	20	45.0	50.0	58.0	57.9	30.3	2.8	0.087329	62504	63073	1118-1282	1.000000	91.3
ycf4_p2	ycf4	2	ATTTCCGGCAATTGCAATGG	CCTCATTCGTCGGCTCTATG	20	20	45.0	55.0	58.0	57.9	30.3	0.0	0.084506	62504	63073	1751-1922	1.000000	89.9
ycf4_p3	ycf4	3	GGATCGCTGGATGGCTAAAA	CCTCATTCGTCGGCTCTATG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.090184	62504	63073	1532-1667	1.000000	87.6
ycf4_p4	ycf4	4	ATTTCCGGCAATTGCAATGG	TCGTCGGCTCTATGTGATCT	20	20	45.0	50.0	58.0	58.0	30.3	0.0	0.058299	62504	63073	1745-1916	0.806452	82.2
ycf4_p5	ycf4	5	GGATCGCTGGATGGCTAAAA	TCGTCGGCTCTATGTGATCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063977	62504	63073	1528-1661	0.806452	79.9
clpP_p1	clpP	1	TAGCTGACCCTCTTAGTCCG	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.140842	72861	74891	2733-2888	0.870968	81.7
clpP_p2	clpP	2	CGTTCTTGACAGAGTGGGAG	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.158299	72861	74891	2715-2870	0.870968	81.6
clpP_p3	clpP	3	AGTCGCACATACACCCTAGT	TTCGTTACAGTCCCTCCTGT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.156718	72861	74891	2885-3000	0.290323	53.6
clpP_p4	clpP	4	AGTCGCACATACACCCTAGT	CCCAGCTACACCTTCGTAAC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.157319	72861	74891	2919-2921	0.193548	46.6
clpP_p5	clpP	5	AGTCGCACATACACCCTAGT	CTGCCAAAAAGCATAAGCCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.165684	72861	74891	2956-2958	0.193548	46.6
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	GACCCAACGGATTCTTCCAA	20	20	50.0	50.0	58.0	57.8	7.6	0.0	0.203402	88959	89395	506-966	1.000000	88.5
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	AAGACCCAACGGATTCTTCC	20	20	50.0	50.0	58.0	57.5	7.6	0.0	0.500876	88959	89395	508-968	1.000000	86.1
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	GGAAGACCCAACGGATTCTT	20	20	50.0	50.0	58.0	57.5	7.6	0.0	0.500876	88959	89395	510-970	1.000000	86.1
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	AGTTGGGCGCTTTAACCATT	GACCCAACGGATTCTTCCAA	20	20	45.0	50.0	58.4	57.8	7.6	0.0	0.566338	88959	89395	507-967	1.000000	85.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TTGGGCGCTTTAACCATTCA	GACCCAACGGATTCTTCCAA	20	20	45.0	50.0	58.4	57.8	7.6	0.0	0.572209	88959	89395	505-965	1.000000	85.5
ycf2_p1	ycf2	1	CCGCTTATGGGGTCAAATCA	AGATCTCGTGCATTGGAACC	20	20	50.0	50.0	57.9	58.0	3.7	0.0	0.081173	93429	93548	1189-1369	1.000000	91.4
ycf2_p2	ycf2	2	ATTGAACAACCGGGAGCAAT	AGATCTCGTGCATTGGAACC	20	20	45.0	50.0	58.1	58.0	6.3	0.0	0.106483	93429	93548	845-1016	1.000000	91.1
ycf2_p3	ycf2	3	AAATCGAATCCTGGGTCGTG	AGATCTCGTGCATTGGAACC	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.054014	93429	93548	1006-1116	0.387097	61.0
ycf2_p4	ycf2	4	AAATCGAATCCTGGGTCGTG	GGTTTAGTTCCGGCAGCATA	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.056856	93429	93548	699-740	0.161290	46.0
ycf2_p5	ycf2	5	CCGCTTATGGGGTCAAATCA	GGTTTAGTTCCGGCAGCATA	20	20	50.0	50.0	57.9	58.0	3.7	0.0	0.084015	93429	93548	882-923	0.161290	45.8
ycf1_p1	ycf1	1	CCCCTTTGGTGTCATTGACA	ACGTCGTGATTTTCCCTAGC	20	20	50.0	50.0	58.0	58.0	15.4	0.0	0.012015	111275	113170	2559-2865	0.387097	65.9
ycf1_p2	ycf1	2	CCCGGAACTTCTTCGTAGTG	ACGTCGTGATTTTCCCTAGC	20	20	55.0	50.0	58.0	58.0	0.7	0.0	0.013801	111275	113170	2668-2974	0.387097	65.6
ycf1_p3	ycf1	3	CCGGAACTTCTTCGTAGTGG	ACGTCGTGATTTTCCCTAGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.013801	111275	113170	2667-2973	0.387097	65.6
ycf1_p4	ycf1	4	GAGCTACTGAGGAACAACGG	GAGATCTTCTTCGGCGGATC	20	20	55.0	55.0	58.0	57.9	0.0	7.2	0.061244	111275	113170	2656-2962	0.387097	65.2
ycf1_p5	ycf1	5	GAGCTACTGAGGAACAACGG	ACGTCGTGATTTTCCCTAGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.008301	111275	113170	2763-2997	0.258065	59.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GTTGCCGTCGGAAAAAGAAG	AAAAATAGCACTTGGGCCGA	20	20	50.0	45.0	58.0	58.1	17.5	0.0	0.114542	116223	117082	1984-2115	1.000000	89.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGCCGTCGGAAAAAGAAGAA	AAAAATAGCACTTGGGCCGA	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.125831	116223	117082	1982-2113	1.000000	89.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GTTGCCGTCGGAAAAAGAAG	TGTCCCGAATAAATCCAGCG	20	20	50.0	50.0	58.0	58.1	17.5	0.0	0.084310	116223	117082	1946-1967	0.677419	71.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGCCGTCGGAAAAAGAAGAA	TGTCCCGAATAAATCCAGCG	20	20	45.0	50.0	58.0	58.1	17.5	0.0	0.095599	116223	117082	1944-1965	0.677419	71.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTGCCGTCGGAAAAAGAAGA	TGTCCCGAATAAATCCAGCG	20	20	45.0	50.0	58.0	58.1	17.5	0.0	0.095599	116223	117082	1945-1966	0.677419	71.4
ycf1_p1	ycf1	1	TCTGTGCCAAGGTTTCAGAC	TGAACGGACCATATCGGAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094191	130248	130367	1103-1256	0.935484	88.7
ycf1_p2	ycf1	2	TGATTATGCCTCGCCGAAAA	TGAACGGACCATATCGGAGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.092768	130248	130367	546-645	0.838710	84.8
ycf1_p3	ycf1	3	TTGATTATGCCTCGCCGAAA	TGAACGGACCATATCGGAGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.092768	130248	130367	547-646	0.774194	82.2
ycf1_p4	ycf1	4	CTTGCCCATATGGAGTGGTC	TGAACGGACCATATCGGAGA	20	20	55.0	50.0	58.0	57.9	24.0	0.0	0.088637	130248	130367	834-852	0.387097	56.6
ycf1_p5	ycf1	5	CTTGCCCATATGGAGTGGTC	CATGCCAAAGTGCTGGAAAG	20	20	55.0	50.0	58.0	57.9	24.0	0.0	0.101675	130248	130367	1128-1146	0.387097	56.1