Hotspot

ycf54

SSC · 109044-109787 · 744 bp

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Pi 0.358
MarkerSeek score 82.40
Primer available yes
Label ycf54

Feature Metadata

label_name
ycf54
feature_type
gene
region
SSC
length_bp
744
variable_sites
340
pi
0.358
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf54_p1 TCCTGAAGTCTGTGCAATCG TCCTGAAGTCTGTGCAATCG 50.0 50.0 57.9 57.9 1913 bp 0.333333 52.0
ycf54_p2 CCCCACATTCTAGCCAAACA TCCTGAAGTCTGTGCAATCG 50.0 50.0 57.8 57.9 1785 bp 0.333333 50.9
ycf54_p3 TCCTGAAGTCTGTGCAATCG CCCCACATTCTAGCCAAACA 50.0 50.0 57.9 57.8 1785 bp 0.333333 50.9
ycf54_p4 CCCCACATTCTAGCCAAACA CCCCACATTCTAGCCAAACA 50.0 50.0 57.8 57.8 1657 bp 0.333333 49.9
ycf54_p5 TCCTGAAGTCTGTGCAATCG ACCATTGACCCGGTTTTAGC 50.0 50.0 57.9 58.5 1944 bp 0.333333 49.0