Hotspot

tatC

LSC · 13578-14324 · 747 bp

Pi 0.446

MarkerSeek score 84.10

Primer available yes

Label tatC

Feature Metadata

label_name: tatC
feature_type: gene
region: LSC
length_bp: 747
variable_sites: 451
pi: 0.446
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tatC_p1	TAAACTTGACCACGACCACG	ACCACAAAGTACAGAAACTGCT	50.0	40.9	58.1	58.1	1680 bp	0.333333	35.3
tatC_p2	TAAACTTGACCACGACCACG	CCACAAAGTACAGAAACTGCTG	50.0	45.5	58.1	57.8	1679 bp	0.333333	34.5
tatC_p3	TAAACTTGACCACGACCACG	ACCACAAAGTACAGAAACTGC	50.0	42.9	58.1	56.7	1680 bp	0.333333	33.5
tatC_p4	AACAGATGTTGGGGCTTCAA	ACCACAAAGTACAGAAACTGCT	45.0	40.9	57.6	58.1	1318 bp	0.333333	33.4
tatC_p5	ACAGATGTTGGGGCTTCAAA	ACCACAAAGTACAGAAACTGCT	45.0	40.9	57.6	58.1	1317 bp	0.333333	33.4