Hotspot

rns-psbD

IRa · 125013-127092 · 2080 bp

Back to Synura

Pi 0.406
MarkerSeek score 73.10
Primer available yes
Label rns-psbD

Feature Metadata

label_name
rns-psbD
feature_type
igs
region
IRa
length_bp
2080
variable_sites
650
pi
0.406
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.63
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rns-psbD_p1 ATTCTCACGCGTTACTCACC TTCAACCGTTGCTCCATGAA 50.0 45.0 58.0 58.0 2853 bp 0.333333 53.1
rns-psbD_p2 ATTCTCACGCGTTACTCACC TTTCAACCGTTGCTCCATGA 50.0 45.0 58.0 58.0 2854 bp 0.333333 53.1
rns-psbD_p3 ATTCTCACGCGTTACTCACC AAAGCTAGGGCATTGTACGG 50.0 50.0 58.0 58.0 2633 bp 0.333333 53.0
rns-psbD_p4 ATTCTCACGCGTTACTCACC TTGGCTGCATCTCCATCTTC 50.0 50.0 58.0 58.0 2885 bp 0.333333 52.9
rns-psbD_p5 ATTCTCACGCGTTACTCACC GCTGCATCTCCATCTTCGAA 50.0 50.0 58.0 58.1 2882 bp 0.333333 52.8