Hotspot

psaC-rnl

IRa · 119480-120319 · 840 bp

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Pi 0.351
MarkerSeek score 75.00
Primer available yes
Label psaC-rnl

Feature Metadata

label_name
psaC-rnl
feature_type
igs
region
IRa
length_bp
840
variable_sites
210
pi
0.351
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.69
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-rnl_p1 GCCTGGGGTTCTTATTGGAG AGTTATCGTGCCAACGGTAC 55.0 50.0 57.9 58.0 1549 bp 0.333333 52.9
psaC-rnl_p2 GCCTGGGGTTCTTATTGGAG TGATCTGACGATGCTGTGTG 55.0 50.0 57.9 58.0 1853 bp 0.333333 52.9
psaC-rnl_p3 GCCTGGGGTTCTTATTGGAG GTGATCTGACGATGCTGTGT 55.0 50.0 57.9 58.0 1854 bp 0.333333 52.9
psaC-rnl_p4 GCCTGGGGTTCTTATTGGAG TCTTTCGGGACTGAAGGAGT 55.0 50.0 57.9 58.0 2102 bp 0.333333 52.8
psaC-rnl_p5 AAGGCTGGCAAGGAGAAAAA AGTTATCGTGCCAACGGTAC 45.0 50.0 57.9 58.0 1455 bp 0.333333 52.6