Hotspot

ccs1-trnN

IRa · 116202-116807 · 606 bp

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Pi 0.422
MarkerSeek score 72.10
Primer available yes
Label ccs1-trnN

Feature Metadata

label_name
ccs1-trnN
feature_type
igs
region
IRa
length_bp
606
variable_sites
133
pi
0.422
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.46
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccs1-trnN_p1 GGTTGGTTTACTTCGATTCCA AACATCGTAGCTGCTCATGG 42.9 50.0 56.2 58.1 1913 bp 0.333333 30.3
ccs1-trnN_p2 GGTTGGTTTACTTCGATTCCA CATCGTAGCTGCTCATGGTT 42.9 50.0 56.2 58.1 1911 bp 0.333333 30.3
ccs1-trnN_p3 GGTTGGTTTACTTCGATTCCA TTCTTCTTAGCAGCTTGGCC 42.9 50.0 56.2 58.2 1835 bp 0.333333 29.3
ccs1-trnN_p4 GGTTGGTTTACTTCGATTCCA ATCCGTGAAACAGCTGAAGG 42.9 50.0 56.2 58.2 1982 bp 0.333333 29.2
ccs1-trnN_p5 TGTTGGGTAGGAGGATTAACA CATCGTAGCTGCTCATGGTT 42.9 50.0 56.0 58.1 1278 bp 0.333333 28.6