Hotspot

rnl-psaC

IRb · 98617-99456 · 840 bp

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Pi 0.351
MarkerSeek score 75.00
Primer available yes
Label rnl-psaC

Feature Metadata

label_name
rnl-psaC
feature_type
igs
region
IRb
length_bp
840
variable_sites
210
pi
0.351
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.69
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rnl-psaC_p1 AGTTATCGTGCCAACGGTAC GCCTGGGGTTCTTATTGGAG 50.0 55.0 58.0 57.9 1549 bp 0.333333 52.9
rnl-psaC_p2 TGATCTGACGATGCTGTGTG GCCTGGGGTTCTTATTGGAG 50.0 55.0 58.0 57.9 1853 bp 0.333333 52.9
rnl-psaC_p3 GTGATCTGACGATGCTGTGT GCCTGGGGTTCTTATTGGAG 50.0 55.0 58.0 57.9 1854 bp 0.333333 52.9
rnl-psaC_p4 TCTTTCGGGACTGAAGGAGT GCCTGGGGTTCTTATTGGAG 50.0 55.0 58.0 57.9 2102 bp 0.333333 52.8
rnl-psaC_p5 AGTTATCGTGCCAACGGTAC AAGGCTGGCAAGGAGAAAAA 50.0 45.0 58.0 57.9 1455 bp 0.333333 52.6