Hotspot

psbD-rns

IRb · 91844-93923 · 2080 bp

Back to Synura

Pi 0.415
MarkerSeek score 73.00
Primer available yes
Label psbD-rns

Feature Metadata

label_name
psbD-rns
feature_type
igs
region
IRb
length_bp
2080
variable_sites
661
pi
0.415
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.62
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-rns_p1 TTCAACCGTTGCTCCATGAA ATTCTCACGCGTTACTCACC 45.0 50.0 58.0 58.0 2853 bp 0.333333 53.1
psbD-rns_p2 TTTCAACCGTTGCTCCATGA ATTCTCACGCGTTACTCACC 45.0 50.0 58.0 58.0 2854 bp 0.333333 53.1
psbD-rns_p3 AAAGCTAGGGCATTGTACGG ATTCTCACGCGTTACTCACC 50.0 50.0 58.0 58.0 2633 bp 0.333333 53.0
psbD-rns_p4 TTGGCTGCATCTCCATCTTC ATTCTCACGCGTTACTCACC 50.0 50.0 58.0 58.0 2885 bp 0.333333 52.9
psbD-rns_p5 GCTGCATCTCCATCTTCGAA ATTCTCACGCGTTACTCACC 50.0 50.0 58.1 58.0 2882 bp 0.333333 52.8