Hotspot

acpP-trnP

LSC · 54539-55740 · 1202 bp

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Pi 0.381
MarkerSeek score 66.70
Primer available yes
Label acpP-trnP

Feature Metadata

label_name
acpP-trnP
feature_type
igs
region
LSC
length_bp
1202
variable_sites
97
pi
0.381
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.17
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
acpP-trnP_p1 GGAACAACTGTACGCGTAGT AAAGCAGACACGCTACCAAA 50.0 45.0 57.9 58.0 494-1479 bp 0.666667 77.9
acpP-trnP_p2 TGTAGGAACAACTGTACGCG AAAGCAGACACGCTACCAAA 50.0 45.0 57.9 58.0 1483 bp 0.333333 52.6
acpP-trnP_p3 GTAGGAACAACTGTACGCGT AAAGCAGACACGCTACCAAA 50.0 45.0 57.9 58.0 1482 bp 0.333333 52.6
acpP-trnP_p4 TGTAGGAACAACTGTACGCG GGGATGACAGGATTCGAACC 50.0 55.0 57.9 58.0 1520 bp 0.333333 52.5
acpP-trnP_p5 GGAACAACTGTACGCGTAGT GGGATGACAGGATTCGAACC 50.0 55.0 57.9 58.0 1516 bp 0.333333 52.5