Hotspot

psbW-clpC

LSC · 45227-45861 · 635 bp

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Pi 0.343
MarkerSeek score 82.70
Primer available yes
Label psbW-clpC

Feature Metadata

label_name
psbW-clpC
feature_type
igs
region
LSC
length_bp
635
variable_sites
290
pi
0.343
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbW-clpC_p1 AATTGGGGTGTAGCCAAGTG CGCCAATTGTAAAGCACCAG 50.0 50.0 58.1 58.0 1789 bp 0.333333 52.6
psbW-clpC_p2 TAGGTTCGAATCCTCCCTCC CGCCAATTGTAAAGCACCAG 55.0 50.0 57.9 58.0 1738 bp 0.333333 52.5
psbW-clpC_p3 AATTGGGGTGTAGCCAAGTG GAACCAGCAACAATTCGAGC 50.0 50.0 58.1 58.0 1422 bp 0.333333 52.5
psbW-clpC_p4 TAGGTTCGAATCCTCCCTCC GAACCAGCAACAATTCGAGC 55.0 50.0 57.9 58.0 1371 bp 0.333333 52.4
psbW-clpC_p5 CCCTCCTCGCCCATTTATTC CGCCAATTGTAAAGCACCAG 55.0 50.0 58.1 58.0 1844 bp 0.333333 52.4