Hotspot

trnH(GUG)-rpl19

LSC · 19034-20348 · 1315 bp

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Pi 0.361
MarkerSeek score 83.10
Primer available yes
Label trnH(GUG)-rpl19

Feature Metadata

label_name
trnH(GUG)-rpl19
feature_type
igs
region
LSC
length_bp
1315
variable_sites
617
pi
0.361
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-rpl19_p1 GCACTTGGAATTGTTGGAGC AACCCAGTCGGATGCATTTT 50.0 45.0 57.9 58.1 2224 bp 0.333333 52.2
trnH(GUG)-rpl19_p2 GCACTTGGAATTGTTGGAGC ACCCAGTCGGATGCATTTTT 50.0 45.0 57.9 58.1 2223 bp 0.333333 52.2
trnH(GUG)-rpl19_p3 TGGCACTTGGAATTGTTGGA AACCCAGTCGGATGCATTTT 45.0 45.0 57.9 58.1 2226 bp 0.333333 52.1
trnH(GUG)-rpl19_p4 TGGCACTTGGAATTGTTGGA ACCCAGTCGGATGCATTTTT 45.0 45.0 57.9 58.1 2225 bp 0.333333 52.1
trnH(GUG)-rpl19_p5 TAAGATTCAGGCGAACGACG ACCCAGTCGGATGCATTTTT 50.0 45.0 58.1 58.1 1787 bp 0.333333 52.0