Hotspot

ycf66

Genome · 79300-80252 · 953 bp

Back to Syntrichia

Pi 0.0307

MarkerSeek score 62.90

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 953
variable_sites: 29
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	AAGTGGTAAGGCAGAGGACT	AGCTAGTCAGGGCGGATAAT	50.0	50.0	58.0	58.0	80-1622 bp	1.0	86.1
ycf66_p2	GCGATATGGCCAAGTGGTAA	AGCTAGTCAGGGCGGATAAT	50.0	50.0	58.0	58.0	80-1633 bp	1.0	86.0
ycf66_p3	CAAGTGGTAAGGCAGAGGAC	AGCTAGTCAGGGCGGATAAT	55.0	50.0	57.9	58.0	80-1623 bp	1.0	85.5
ycf66_p4	AAGTGGTAAGGCAGAGGACT	AACAAGCCTTGGTGGTGAAA	50.0	45.0	58.0	58.1	80-2014 bp	1.0	84.4
ycf66_p5	GCGATATGGCCAAGTGGTAA	AACAAGCCTTGGTGGTGAAA	50.0	45.0	58.0	58.1	80-2025 bp	1.0	84.3