Hotspot

ycf2

Genome · 46711-53460 · 6750 bp

Back to Syntrichia

Pi 0.0236

MarkerSeek score 54.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6750
variable_sites: 142
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AGGAGAAAAAGCCATCTTTGA	ACTCAGAATGGTGGAAAATTTGG	38.1	39.1	55.4	57.5	80-1091 bp	1.0	49.2
ycf2_p2	AGGAGAAAAAGCCATCTTTGA	TGAAAACTCAGAATGGTGGA	38.1	40.0	55.4	54.5	80-1096 bp	1.0	49.2
ycf2_p3	AGGAGAAAAAGCCATCTTTGA	TGAAAACTCAGAATGGTGGAA	38.1	38.1	55.4	55.3	80-1096 bp	1.0	49.2
ycf2_p4	AGGAGAAAAAGCCATCTTTGA	TTGAAAACTCAGAATGGTGGA	38.1	38.1	55.4	55.3	80-1097 bp	1.0	49.2
ycf2_p5	AGGAGAAAAAGCCATCTTTGA	CTCAGAATGGTGGAAAATTTGGA	38.1	39.1	55.4	57.3	80-1090 bp	1.0	49.2