Hotspot

ycf1

Genome · 97374-102050 · 4677 bp

Back to Syntrichia

Pi 0.0269

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4677
variable_sites: 126
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGGGGTGACGATTGGAATG	TGAAATTTGTTCCGGTAAGCT	50.0	38.1	57.9	56.1	80-601 bp	1.0	62.9
ycf1_p2	GAATGGGGTGACGATTGGAA	TGAAATTTGTTCCGGTAAGCT	50.0	38.1	57.9	56.1	80-603 bp	1.0	62.4
ycf1_p3	ATGGGGTGACGATTGGAATG	TGAAATTTGTTCCGGTAAGCTT	50.0	36.4	57.9	56.8	80-601 bp	1.0	60.8
ycf1_p4	GCAATAGCGAATCGATGGGA	TGAAATTTGTTCCGGTAAGCT	50.0	38.1	58.2	56.1	80-750 bp	1.0	60.5
ycf1_p5	CGTCTTGCAATAGCGAATCG	TGAAATTTGTTCCGGTAAGCT	50.0	38.1	57.7	56.1	80-756 bp	1.0	60.0