Hotspot

rpl23

Genome · 358-633 · 276 bp

Back to Syntrichia

Pi 0.0254

MarkerSeek score 52.20

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 276
variable_sites: 7
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	AAGCATCCATAGCTGAACGG	ACCTCCTCCTCTATAACGGC	50.0	55.0	58.0	58.0	773-775 bp	1.0	82.2
rpl23_p2	TGAACGGTTAAAGCACCCAA	ACCTCCTCCTCTATAACGGC	45.0	55.0	57.9	58.0	760-762 bp	1.0	82.2
rpl23_p3	AACGGTTAAAGCACCCAACT	ACCTCCTCCTCTATAACGGC	45.0	55.0	57.9	58.0	758-760 bp	1.0	82.1
rpl23_p4	AAGCATCCATAGCTGAACGG	AAACGTTTGTGACCTCCTCC	50.0	50.0	58.0	58.0	784-786 bp	1.0	81.9
rpl23_p5	TGAACGGTTAAAGCACCCAA	AAACGTTTGTGACCTCCTCC	45.0	50.0	57.9	58.0	771-773 bp	1.0	81.9