Hotspot

orf40

Genome · 19422-20105 · 684 bp

Back to Syntrichia

Pi 0.0336

MarkerSeek score 65.60

Primer available yes

Label orf40

Feature Metadata

label_name: orf40
feature_type: gene
region: Genome
length_bp: 684
variable_sites: 23
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf40_p1	GGCAAATACCACCGGAAGAA	TGTTGATAGTGTGCCGTCAG	50.0	50.0	58.2	57.9	1396-1398 bp	1.0	80.2
orf40_p2	AATGGCAAATACCACCGGAA	TGTTGATAGTGTGCCGTCAG	45.0	50.0	57.8	57.9	1399-1401 bp	1.0	79.8
orf40_p3	AAATGGCAAATACCACCGGA	TGTTGATAGTGTGCCGTCAG	45.0	50.0	57.8	57.9	1400-1402 bp	1.0	79.7
orf40_p4	ATGGCAAATACCACCGGAAG	TGTTGATAGTGTGCCGTCAG	50.0	50.0	58.2	57.9	1398-1400 bp	1.0	79.6
orf40_p5	GGCAAATACCACCGGAAGAA	GTAATTGGTCCTGTTCCCGG	50.0	55.0	58.2	58.3	1662-1664 bp	1.0	78.5