Hotspot

ndhB

Genome · 80922-83063 · 2142 bp

Back to Syntrichia

Pi 0.0206

MarkerSeek score 57.30

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2142
variable_sites: 44
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATTATCCGCCCTGACTAGCT	GCCTCCAGAAAACGTTTTGG	50.0	50.0	58.0	57.9	80-2949 bp	1.0	82.9
ndhB_p2	TTTCACCACCAAGGCTTGTT	ACGTGTAGGTGGCTCTACTT	45.0	50.0	58.1	58.1	80-2642 bp	1.0	82.5
ndhB_p3	TTTCACCACCAAGGCTTGTT	AAAGCAAGACGTGTAGGTGG	45.0	50.0	58.1	58.1	80-2650 bp	1.0	82.1
ndhB_p4	ATTATCCGCCCTGACTAGCT	CGCCTCCAGAAAACGTTTTG	50.0	50.0	58.0	58.2	80-2950 bp	1.0	82.0
ndhB_p5	TTTCACCACCAAGGCTTGTT	GTCACGTCGTAGTACTGCAG	45.0	55.0	58.1	58.1	80-2873 bp	1.0	81.9