Hotspot

trnS-GCU-psaM

Genome · 61098-61370 · 273 bp

Back to Syntrichia

Pi 0.059
MarkerSeek score 71.20
Primer available yes
Label trnS-GCU-psaM

Feature Metadata

label_name
trnS-GCU-psaM
feature_type
igs
region
Genome
length_bp
273
variable_sites
16
pi
0.059
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psaM_p1 TTAAACCACTCGGCCATCTC GGTATTTTGGCTTTAAGATTAGGT 50.0 33.3 57.6 55.1 80-338 bp 1.0 46.9
trnS-GCU-psaM_p2 TAAACCACTCGGCCATCTCT GGTATTTTGGCTTTAAGATTAGGT 50.0 33.3 58.4 55.1 80-337 bp 1.0 46.9
trnS-GCU-psaM_p3 CATCGCTTTAAACCACTCGG GGTATTTTGGCTTTAAGATTAGGT 50.0 33.3 57.2 55.1 80-345 bp 1.0 46.8
trnS-GCU-psaM_p4 CCATCGCTTTAAACCACTCG GGTATTTTGGCTTTAAGATTAGGT 50.0 33.3 57.2 55.1 80-346 bp 1.0 46.8
trnS-GCU-psaM_p5 GCTTTAAACCACTCGGCCAT GGTATTTTGGCTTTAAGATTAGGT 50.0 33.3 58.8 55.1 80-341 bp 1.0 46.8