Hotspot

psbI-trnS-GCU

Genome · 60911-61011 · 101 bp

Back to Syntrichia

Pi 0.0714

MarkerSeek score 74.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 101
variable_sites: 7
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TGGTCGTAATCCTGGACGTA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	80-151 bp	1.0	84.2
psbI-trnS-GCU_p2	GCTGTTCGTTTACACAGTGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.3	58.2	80-220 bp	1.0	78.3
psbI-trnS-GCU_p3	TGGTCGTAATCCTGGACGT	GGGTTCGAATCCCTCTCTCT	52.6	55.0	58.0	58.2	80-151 bp	1.0	76.9
psbI-trnS-GCU_p4	TGGTCGTAATCCTGGACGTA	GGTTCGAATCCCTCTCTCTC	50.0	55.0	57.9	56.8	80-150 bp	1.0	76.6
psbI-trnS-GCU_p5	TGGTCGTAATCCTGGACGTA	GTTCGAATCCCTCTCTCTCC	50.0	55.0	57.9	56.8	80-149 bp	1.0	76.6