Hotspot

psbD-trnT-GGU

Genome · 45351-45780 · 430 bp

Back to Syntrichia

Pi 0.0372

MarkerSeek score 65.90

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 430
variable_sites: 16
pi: 0.0372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AAAACGGTCTCTTCGTAGCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.9	58.3	549-573 bp	1.0	79.4
psbD-trnT-GGU_p2	TCTTCGTAGCCAGTCATCCA	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	540-564 bp	1.0	78.8
psbD-trnT-GGU_p3	AAAACGGTCTCTTCGTAGCC	TGGTAGAGTAACGCCATGGT	50.0	50.0	57.9	58.4	571-595 bp	1.0	78.1
psbD-trnT-GGU_p4	TCTTCGTAGCCAGTCATCCA	TGGTAGAGTAACGCCATGGT	50.0	50.0	58.2	58.4	562-586 bp	1.0	77.5
psbD-trnT-GGU_p5	AAAACGGTCTCTTCGTAGCC	GCCATGGTAAGGCGTAAGTC	50.0	55.0	57.9	58.7	559-583 bp	1.0	76.0