Hotspot

ycf4-psaI

Genome · 23496-23848 · 353 bp

Back to Syntrichia

Pi 0.0516

MarkerSeek score 70.30

Primer available yes

Label ycf4-psaI

Feature Metadata

label_name: ycf4-psaI
feature_type: igs
region: Genome
length_bp: 353
variable_sites: 18
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-psaI_p1	CCAAAGCCATTCAGATTCACG	ACCTTCGATTTTTGTGCCTT	47.6	40.0	57.9	56.1	474-494 bp	1.0	59.3
ycf4-psaI_p2	CCAAAGCCATTCAGATTCACG	ACCTTCGATTTTTGTGCCTTT	47.6	38.1	57.9	56.9	474-494 bp	1.0	57.4
ycf4-psaI_p3	CCAAAGCCATTCAGATTCACG	TCCAGCAATTACAATGGCTTC	47.6	42.9	57.9	56.9	438-458 bp	1.0	57.2
ycf4-psaI_p4	CCAAAGCCATTCAGATTCACG	CCAGCAATTACAATGGCTTCT	47.6	42.9	57.9	56.8	437-457 bp	1.0	57.2
ycf4-psaI_p5	CCAAAGCCATTCAGATTCACG	TCCAGCAATTACAATGGCTTCT	47.6	40.9	57.9	58.3	438-458 bp	1.0	56.0