Hotspot

cemA-ycf4

Genome · 22833-22940 · 108 bp

Back to Syntrichia

Pi 0.0926

MarkerSeek score 73.10

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: Genome
length_bp: 108
variable_sites: 10
pi: 0.0926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	TGGTGCACTATATGATTTCAT	GCAGCAGAGTTAGCTCGATT	33.3	50.0	52.7	58.1	177 bp	1.0	44.5
cemA-ycf4_p2	TGGTGCACTATATGATTTCAT	AGCAGCAGAGTTAGCTCGAT	33.3	50.0	52.7	58.9	178 bp	1.0	44.5
cemA-ycf4_p3	TGGTGCACTATATGATTTCAT	AAGCAGCAGAGTTAGCTCGA	33.3	50.0	52.7	59.1	179 bp	1.0	44.5
cemA-ycf4_p4	TGGTGCACTATATGATTTCAT	AGCAGCAGAGTTAGCTCGA	33.3	52.6	52.7	58.4	178 bp	1.0	44.5
cemA-ycf4_p5	TGGTGCACTATATGATTTCAT	AAAGCAGCAGAGTTAGCTCG	33.3	50.0	52.7	58.3	180 bp	1.0	44.4