Hotspot

psbN-psbT

Genome · 10475-10572 · 98 bp

Back to Syntrichia

Pi 0.153

MarkerSeek score 84.90

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: Genome
length_bp: 98
variable_sites: 15
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	CAGCTTTTGGACAACCCTCT	TTTTCGTGAACCGCCTACAA	50.0	45.0	58.0	58.0	200-202 bp	1.0	85.5
psbN-psbT_p2	CAGCTTTTGGACAACCCTCT	TTTTTCGTGAACCGCCTACA	50.0	45.0	58.0	58.0	201-203 bp	1.0	85.4
psbN-psbT_p3	CAGCTTTTGGACAACCCTCT	TTCGTGAACCGCCTACAATT	50.0	45.0	58.0	57.8	198-200 bp	1.0	84.5
psbN-psbT_p4	CAGCTTTTGGACAACCCTCT	TTTCGTGAACCGCCTACAAT	50.0	45.0	58.0	57.8	199-201 bp	1.0	84.5
psbN-psbT_p5	CAGCTTTTGGACAACCCTCT	TCGTGAACCGCCTACAATTC	50.0	50.0	58.0	58.3	197-199 bp	1.0	83.7