Hotspot

ycf1

SSC · 121999-127071 · 5073 bp

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Pi 0.0023

MarkerSeek score 40.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5073
variable_sites: 20
pi: 0.0023
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCTTTTCCGTTTGAGTTTCAC	ACCGTTTTGGGAATGGAAAC	40.9	45.0	57.4	56.8	272 bp	1.0	50.3
ycf1_p2	CCTTTTCCGTTTGAGTTTCACT	ACCGTTTTGGGAATGGAAAC	40.9	45.0	57.4	56.8	271 bp	1.0	50.3
ycf1_p3	TCCGTTTGAGTTTCACTTGA	ACCGTTTTGGGAATGGAAAC	40.0	45.0	55.2	56.8	266 bp	1.0	48.6
ycf1_p4	TCCTTTTCCGTTTGAGTTTCAC	AACCGTTTTGGGAATGGAAAC	40.9	42.9	57.4	57.5	273 bp	1.0	48.2
ycf1_p5	CCTTTTCCGTTTGAGTTTCACT	AACCGTTTTGGGAATGGAAAC	40.9	42.9	57.4	57.5	272 bp	1.0	48.1