Hotspot

matK

LSC · 2075-3586 · 1512 bp

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Pi 0.00143
MarkerSeek score 59.70
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1512
variable_sites
4
pi
0.00143
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TCCTTACTACCTCAACATTTCAGA CCTTGTTTTGACTGTATCGCA 37.5 42.9 57.1 56.8 1694-1695 bp 1.0 40.2
matK_p2 TCCTTACTACCTCAACATTTCAGA ACCTTGTTTTGACTGTATCGC 37.5 42.9 57.1 56.8 1695-1696 bp 1.0 40.2
matK_p3 TCCTTACTACCTCAACATTTCAGA CCTTGTTTTGACTGTATCGCAC 37.5 45.5 57.1 58.2 1694-1695 bp 1.0 40.2
matK_p4 TCCTTACTACCTCAACATTTCAGA ACCTTGTTTTGACTGTATCGCA 37.5 40.9 57.1 58.5 1695-1696 bp 1.0 40.2
matK_p5 CCTTACTACCTCAACATTTCAGA CCTTGTTTTGACTGTATCGCA 39.1 42.9 55.7 56.8 1693-1694 bp 1.0 40.2