Hotspot

rpl32-trnL-UAG

SSC · 111713-112508 · 796 bp

Back to Synotis

Pi 0.0136
MarkerSeek score 68.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
796
variable_sites
18
pi
0.0136
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 913-927 bp 1.0 80.8
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 914-928 bp 1.0 80.8
rpl32-trnL-UAG_p3 AAAGGATATTGGGCAGCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 924-938 bp 1.0 80.7
rpl32-trnL-UAG_p4 AAAGGATATTGGGCAGCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 925-939 bp 1.0 80.7
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 923-937 bp 1.0 79.8