Hotspot

trnR-UCU-trnG-UCC

LSC · 29975-30218 · 244 bp

Back to Synotis

Pi 0.0041

MarkerSeek score 55.90

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 2
pi: 0.0041
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	323-329 bp	1.0	64.3
trnR-UCU-trnG-UCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	326-332 bp	1.0	64.1
trnR-UCU-trnG-UCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	329-335 bp	1.0	63.6
trnR-UCU-trnG-UCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	332-338 bp	1.0	63.4
trnR-UCU-trnG-UCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	327-333 bp	1.0	61.9