Hotspot

trnE-UUC-rpoB

LSC · 12081-13030 · 950 bp

Back to Synotis

Pi 0.00299

MarkerSeek score 61.20

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 5
pi: 0.00299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGAGTTGAACCCTCGTTTT	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.0	57.8	1091-1110 bp	1.0	78.5
trnE-UUC-rpoB_p2	GGGAGTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCCG	50.0	50.0	58.0	57.7	1092-1111 bp	1.0	78.4
trnE-UUC-rpoB_p3	TAACCACTGGACCATAGGGG	GTTCTTCTGTCAAACCCCGA	55.0	50.0	58.1	57.8	1050-1069 bp	1.0	77.8
trnE-UUC-rpoB_p4	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCCG	55.0	50.0	58.1	57.7	1051-1070 bp	1.0	77.7
trnE-UUC-rpoB_p5	CCCTCGTTTTCTCCGTGAAA	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.1	57.8	1081-1100 bp	1.0	77.7