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psbM-trnD-GUC

LSC · 10952-11577 · 626 bp

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Pi 0.00426

MarkerSeek score 52.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 4
pi: 0.00426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.6	57.1	742-743 bp	1.0	45.9
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCTAGAGCACCG	40.0	50.0	54.5	57.1	736-737 bp	1.0	45.5
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.4	57.1	739-740 bp	1.0	44.5
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	CAATAGGCTAGAGCACCGC	39.1	57.9	57.6	58.1	740-741 bp	1.0	44.3
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.3	57.1	743-744 bp	1.0	43.8