Hotspot

rps16

LSC · 5274-6725 · 1452 bp

Back to Syngonium

Pi 0.00694

MarkerSeek score 50.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1452
variable_sites: 10
pi: 0.00694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGGGTGGGGATAAACAGAT	ATCAATTCCATCCCGGTTCG	50.0	50.0	57.7	58.0	1910-1927 bp	1.0	78.3
rps16_p2	ATTGCACGATCCCACCAAAA	ATCAATTCCATCCCGGTTCG	45.0	50.0	58.4	58.0	2161-2165 bp	1.0	78.1
rps16_p3	AGGGGTGGGGATAAACAGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	2590-2605 bp	1.0	77.8
rps16_p4	AGGGGTGGGGATAAACAGAT	ATCCTTCCGTCCCAGATCAA	50.0	50.0	57.7	58.1	2581-2596 bp	1.0	77.4
rps16_p5	AGGGGTGGGGATAAACAGAT	TCCTTCCGTCCCAGATCAAT	50.0	50.0	57.7	58.1	2580-2595 bp	1.0	77.4