Hotspot

petD

LSC · 82310-83538 · 1229 bp

Back to Syngonium

Pi 0.00407

MarkerSeek score 46.90

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1229
variable_sites: 5
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGCAGGGTATTTCAGGTCCT	CATGCCGAGGAAAAGGACAT	50.0	50.0	58.0	58.2	2075-2078 bp	1.0	79.6
petD_p2	TGGGTTACGGGTGTAGTTCT	CATGCCGAGGAAAAGGACAT	50.0	50.0	58.0	58.2	2346-2349 bp	1.0	79.5
petD_p3	ATTGCCTCTTCTTACTGCCG	CATGCCGAGGAAAAGGACAT	50.0	50.0	58.0	58.2	2128-2131 bp	1.0	79.5
petD_p4	CAGTTCATCGATGGTCAGCA	CATGCCGAGGAAAAGGACAT	50.0	50.0	58.0	58.2	2447-2450 bp	1.0	79.4
petD_p5	AGTTGAAGTGGATCCTCCGA	CATGCCGAGGAAAAGGACAT	50.0	50.0	58.1	58.2	1877-1881 bp	1.0	79.4