Hotspot

rps15-ycf1

SSC · 132314-133281 · 968 bp

Back to Syngonium

Pi 0.00436

MarkerSeek score 66.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 968
variable_sites: 4
pi: 0.00436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCGACAGACCCTTTGTTTTCT	AGCTTGTATGAATCGGTATTGGT	42.9	39.1	57.4	58.0	1130-1157 bp	1.0	47.1
rps15-ycf1_p2	TCGACAGACCCTTTGTTTTCT	TGGTAGTCGTTTCAGTATGTCA	42.9	40.9	57.4	56.9	1094-1121 bp	1.0	46.6
rps15-ycf1_p3	ACTTGAAATTCGACAGACCCT	AGCTTGTATGAATCGGTATTGGT	42.9	39.1	57.2	58.0	1139-1166 bp	1.0	45.4
rps15-ycf1_p4	ACTTGAAATTCGACAGACCCT	TGGTAGTCGTTTCAGTATGTCA	42.9	40.9	57.2	56.9	1103-1130 bp	1.0	44.8
rps15-ycf1_p5	TCGACAGACCCTTTGTTTTCT	GCTTGTATGAATCGGTATTGGT	42.9	40.9	57.4	56.6	1129-1156 bp	1.0	44.0