Hotspot

psaC-ndhE

SSC · 125360-126227 · 868 bp

Back to Syngonium

Pi 0.0049

MarkerSeek score 66.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 868
variable_sites: 4
pi: 0.0049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTTTACATCCTGGCCAAG	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.9	57.8	994-1027 bp	1.0	73.9
psaC-ndhE_p2	AGCTTTACATCCTGGCCAAG	TGAAGCAGCTATTGGTCTAGC	50.0	47.6	57.9	57.8	1034-1067 bp	1.0	73.7
psaC-ndhE_p3	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	55.0	42.9	58.4	57.8	946-979 bp	1.0	72.2
psaC-ndhE_p4	GGCCCGTACACATTGAGTAC	TGAAGCAGCTATTGGTCTAGC	55.0	47.6	58.4	57.8	986-1019 bp	1.0	72.0
psaC-ndhE_p5	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.6	57.8	945-978 bp	1.0	72.0