Hotspot

tRNA-Arg(ACG)-tRNA-Asn(GUU)

IRb · 115601-116185 · 585 bp

Back to Syngonium

Pi 0.00518

MarkerSeek score 62.40

Primer available yes

Label tRNA-Arg(ACG)-tRNA-Asn(GUU)

Feature Metadata

label_name: tRNA-Arg(ACG)-tRNA-Asn(GUU)
feature_type: igs
region: IRb
length_bp: 585
variable_sites: 3
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Arg(ACG)-tRNA-Asn(GUU)_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	720-726 bp	1.0	79.2
tRNA-Arg(ACG)-tRNA-Asn(GUU)_p2	CAGAGGATTAGAGCACGTGG	CACCCGTTGTTCCTCAGTAG	55.0	55.0	57.8	57.9	723-729 bp	1.0	78.6
tRNA-Arg(ACG)-tRNA-Asn(GUU)_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	671-677 bp	1.0	78.1
tRNA-Arg(ACG)-tRNA-Asn(GUU)_p4	CAGAGGATTAGAGCACGTGG	GAATCACCCGTTGTTCCTCA	55.0	50.0	57.8	57.8	727-733 bp	1.0	77.8
tRNA-Arg(ACG)-tRNA-Asn(GUU)_p5	CAGAGGATTAGAGCACGTGG	AATCACCCGTTGTTCCTCAG	55.0	50.0	57.8	57.8	726-732 bp	1.0	77.8