Hotspot

tRNA-Leu(CAA)-ndhB

IRb · 100859-102010 · 1152 bp

Back to Syngonium

Pi 0.00189

MarkerSeek score 63.80

Primer available yes

Label tRNA-Leu(CAA)-ndhB

Feature Metadata

label_name: tRNA-Leu(CAA)-ndhB
feature_type: igs
region: IRb
length_bp: 1152
variable_sites: 2
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu(CAA)-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1140-1212 bp	1.0	83.6
tRNA-Leu(CAA)-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1138-1210 bp	1.0	83.6
tRNA-Leu(CAA)-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	1135-1207 bp	1.0	82.1
tRNA-Leu(CAA)-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	1139-1211 bp	1.0	81.5
tRNA-Leu(CAA)-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	1137-1209 bp	1.0	81.3