Hotspot

rps19-rpl2

IRb · 90699-90752 · 54 bp

Back to Syngonium

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 54
variable_sites: 1
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGATGCCCTAGACCAAGTT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.3	58.0	252 bp	1.0	77.5
rps19-rpl2_p2	TGGATGCCCTAGACCAAGTT	CGTCGCCGTAACCGTAAATA	50.0	50.0	58.3	58.2	190 bp	1.0	76.3
rps19-rpl2_p3	TGGATGCCCTAGACCAAGTT	TATTCTTCGTCGCCGTAACC	50.0	50.0	58.3	57.8	197 bp	1.0	76.2
rps19-rpl2_p4	TGGATGCCCTAGACCAAGTT	TATCCTGCGCTTGGAAGAAG	50.0	50.0	58.3	57.7	250 bp	1.0	75.1
rps19-rpl2_p5	TGGATGCCCTAGACCAAGTT	CTGCGCTTGGAAGAAGAAGT	50.0	50.0	58.3	58.5	246 bp	1.0	73.8