Hotspot

petD-rpoA

LSC · 83539-83822 · 284 bp

Back to Syngonium

Pi 0.05

MarkerSeek score 77.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 13
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTTTTGATCGGTACCGCAGT	AGTGGACATTCTACAGAAGCA	45.0	42.9	57.8	56.7	432-436 bp	1.0	66.3
petD-rpoA_p2	GGTACCGCAGTAGCTCTTTG	AGTGGACATTCTACAGAAGCA	55.0	42.9	58.4	56.7	423-427 bp	1.0	64.9
petD-rpoA_p3	GTACCGCAGTAGCTCTTTGG	AGTGGACATTCTACAGAAGCA	55.0	42.9	58.4	56.7	422-426 bp	1.0	64.9
petD-rpoA_p4	CCGCAGTAGCTCTTTGGTTA	AGTGGACATTCTACAGAAGCA	50.0	42.9	57.6	56.7	419-423 bp	1.0	64.7
petD-rpoA_p5	TCTTTTTGATCGGTACCGCA	AGTGGACATTCTACAGAAGCA	45.0	42.9	57.5	56.7	434-438 bp	1.0	64.0