Hotspot

ndhC-tRNA-Val(UAC)

LSC · 55317-56641 · 1325 bp

Back to Syngonium

Pi 0.00607

MarkerSeek score 62.80

Primer available yes

Label ndhC-tRNA-Val(UAC)

Feature Metadata

label_name: ndhC-tRNA-Val(UAC)
feature_type: igs
region: LSC
length_bp: 1325
variable_sites: 8
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Val(UAC)_p1	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1450-1457 bp	1.0	77.7
ndhC-tRNA-Val(UAC)_p2	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1452-1459 bp	1.0	75.9
ndhC-tRNA-Val(UAC)_p3	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1453-1460 bp	1.0	75.9
ndhC-tRNA-Val(UAC)_p4	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1462-1469 bp	1.0	75.6
ndhC-tRNA-Val(UAC)_p5	CGGGGCTAAAACTCCAGAAA	AGCCCAATGCAACAAAATGC	50.0	45.0	57.8	58.5	1532-1539 bp	1.0	75.5