Hotspot

rps4-tRNA-Thr(UGU)

LSC · 49435-50110 · 676 bp

Back to Syngonium

Pi 0.0398
MarkerSeek score 73.80
Primer available yes
Label rps4-tRNA-Thr(UGU)

Feature Metadata

label_name
rps4-tRNA-Thr(UGU)
feature_type
igs
region
LSC
length_bp
676
variable_sites
25
pi
0.0398
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-tRNA-Thr(UGU)_p1 CCGAGCGCAATTGGTTTTTA GATGGTCATCGGTTCGACTC 45.0 55.0 57.7 58.2 779-823 bp 1.0 82.5
rps4-tRNA-Thr(UGU)_p2 TCACTTCTGGATCTGGGTCT GATGGTCATCGGTTCGACTC 50.0 55.0 57.4 58.2 756-800 bp 1.0 80.3
rps4-tRNA-Thr(UGU)_p3 AGTCAGTCCTGGTAAAGCCC GATGGTCATCGGTTCGACTC 55.0 55.0 59.0 58.2 730-774 bp 1.0 77.3
rps4-tRNA-Thr(UGU)_p4 TCACTTCTGGATCTGGGTCTT GATGGTCATCGGTTCGACTC 47.6 55.0 58.1 58.2 756-800 bp 1.0 76.6
rps4-tRNA-Thr(UGU)_p5 ATCACTTCTGGATCTGGGTCT GATGGTCATCGGTTCGACTC 47.6 55.0 57.9 58.2 757-801 bp 1.0 76.3