Hotspot

tRNA-Cys(GCA)-petN

LSC · 30128-31008 · 881 bp

Back to Syngonium

Pi 0.00568

MarkerSeek score 62.10

Primer available yes

Label tRNA-Cys(GCA)-petN

Feature Metadata

label_name: tRNA-Cys(GCA)-petN
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 5
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Cys(GCA)-petN_p1	TTTTGTTTTGGCGACATGGC	AGTCCACTCCTTCCCCATAC	45.0	55.0	58.4	58.1	1046-1047 bp	1.0	76.1
tRNA-Cys(GCA)-petN_p2	TTTTGTTTTGGCGACATGGC	GTCCACTCCTTCCCCATACT	45.0	55.0	58.4	58.1	1045-1046 bp	1.0	76.1
tRNA-Cys(GCA)-petN_p3	TTTTGTTTTGGCGACATGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.4	58.2	987-988 bp	1.0	75.9
tRNA-Cys(GCA)-petN_p4	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.5	58.1	988-989 bp	1.0	75.7
tRNA-Cys(GCA)-petN_p5	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	50.0	55.0	58.5	58.1	987-988 bp	1.0	75.7